Fig. 3: Performance of DELFI analyses for lung cancer patients and non-cancer individuals.

a DELFI score distribution across non-cancer individuals and cancer patients, stratified by stage and histology groups in the LUCAS cohort. The box-plot shows the median DELFI score and the inter-quartile range with the individual sample values overlaid as dots. The non-cancer cases with or without benign lesions have a lower DELFI score compared to cancer cases and there is a stepwise increase in DELFI score by stage. The highest median DELFI score is observed in SCLC cases. Green curves indicate all individuals in the LUCAS cohort, orange represents patients without prior history of cancer, and blue indicates patients without prior history of cancer, age 50–80, and with ≥20 pack-year smoking history. The center line in the boxplots represents the median, the upper limit of the boxplots represents the third quantile (75th percentile), the lower limit of the boxplots represents the first quantile (25th percentile), the upper whiskers is the maximum value of the data that is within 1.5 times the interquartile range over the 75th percentile, and the lower whisker is the minimum value of the data that is within 1.5 times the interquartile range under the 25th percentile. b ROC analyses of the overall LUCAS cohort as well as by stage and histology. The dotted vertical line in the ROC figures represents an 80% specificity as a decision boundary. c Analysis of a DELFI fixed model and score cutoff of 0.344 determined from the LUCAS cohort was applied in the validation cohort. The performance of this classifier in the independent cohort was similar to LUCAS in both specificity (left) and sensitivity (right) across all tumor stages. The number of samples in the training and validation sets are indicated in the labels of the horizontal axis. The intervals presented reflect a 90% confidence interval. Additional analyses at other specificities are indicated in Supplementary Fig. 6.