Fig. 2: Pf-derived EVs inhibit the translation of CXCL10.
From: Malaria parasites both repress host CXCL10 and use it as a cue for growth acceleration
A Polysomal profiling of THP-1 cells 14 h post transfection with poly(dA:dT) (blue), Pf –derived EVs (purple), or not treated (NT) (black). One representative repeat out of five biological replicates is shown. B. THP-1 cells were incubated with Pf-ring-stage-derived EVs or transfected with poly(dA:dT) for 14 h or NT. Polysomal profiling was performed on all samples and RNA was isolated from heavy, light and free fractions. Real-time PCR for CXCL10 was performed on all the polysome fractions. n = 3 biologically independent experiments, NT (not treated) THP-1 cells were used as control. One-way ANOVA followed by Dunnett’s test for the heavy fractions (dAdT - NT ***P = 1.64e-05, Pf EVs - NT *P = 0.0368). C. THP-1 cells were incubated with Pf-ring-stage-derived EVs, transfected with poly(dA:dT) for 14 h or NT. Ribosomal profiling was performed on all samples and RNA was isolated from heavy, light and free fractions. Real-time PCR for IFNB was performed on all the polysome fractions. n = 3 biologically independent experiments. One-way ANOVA followed by Dunnett’s test for the heavy fractions (dAdT - NT ***P = 3.23e-05, Pf EVs - NT ***P = 0.000543). Source data are provided as a Source Data file.
