Table 1 Sequence and insertion-deletion length of THP-1 knock-out genotypes for RIG-I, cGAS, and MAVS.
From: Malaria parasites both repress host CXCL10 and use it as a cue for growth acceleration
Cell line | InDel allele 1 | Sequence allele 1 | Indel allele 2 | Sequence allele 2 |
|---|---|---|---|---|
THP-1 MAVS−/− #1 | −23bp | CCAGCG…CTGGG | Homozygous | – |
THP-1 MAVS−/− #2 | 1 bp | CAGCGG.CATCAGG | Homozygous | – |
THP-1 RIG-I (DDX58)−/− #1 | −5bp | CCGGAT…CCTGGAAG | −1 bp | GATATA.TCCTGGA |
THP-1 RIG-I (DDX58)−/− #2 | −4bp | GATATAA…GGAAGGC | −5 bp | ATATAAT…AAGGCTT |
THP-1 CGAS−/− #1 | −1bp | CCCCAGT.GCGCGGAC | +1 bp | CCCCAGT[+T]TGCGCGGAC |
THP-1 CGAS−/− #2 | −1bp | CCCCAGT.GCGCGGAC | −49 bp | AGGCCG[+GCGGGTCTCGA CCCCCGTTCGCCTAGG]…GCGCCCC |
