Fig. 3: Correlative ultrastructure and ionoluminescence mapping towards quantitative localization of single nanodiamonds in a whole HeLa cell.
From: Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy
a Demonstration of correlative ultrastructure and ionoluminescence imaging of the HeLa cell with endocytosed nanodiamonds, realized in a single imaging experiment. The basic experimental design (top right) enables simultaneous acquisition of luminescence image of the nanodiamonds and 3D rendering of cell ultrastructure, by capturing α-particle-induced photons with a photomultiplier tube (PMT) and by detecting the energy loss (ΔE) of the transmitted α-particles through a Si surface barrier detector, respectively. b Overlay of the structural and luminescent images presented in a, showing localization of nanodiamonds in the cell. Scale bar, 2 μm. c Measurement of the number of intracellular nanodiamonds displayed in b in terms of their distance to the cell nuclear boundary.
