Fig. 3: MAIT cell expansion via 5-OP-RU treatment results in enhanced anti-tumor immunity.
a Schematic of experimental setup. b Proportion and absolute number of MAIT cells at day 6 post treatment following intranasal administration of 50 µL of 233 μM 5-OP-RU or PBS at days 0, 1, 2, and 4 in B6-MAITcast MR1 WT mice. Data is presented as representative flow cytometry plots and mean ± SEM of n = 16 independent mice per group from four combined experiments, two-tailed Mann–Whitney test. c Expression of indicated cell surface markers on MAIT cells following intranasal treatment of mice as per (a). Data is presented as mean ± SEM of n = 7 per group from two combined experiments, two-tailed Mann–Whitney test. d Following treatment of mice as per (a). 2 × 105 B16F10 tumor cells were injected i.v. into B6-MAITcast MR1 WT mice. Tumor cell metastases were enumerated from lungs 14 days post tumor inoculation. Data is presented as mean ± SEM of n = 19 (non-treated) and n = 24 (5-OP-RU treated) independent mice from four combined experiments, two-tailed Mann–Whitney test. e Following treatment of mice as per (a). 2 × 105 B16F10 tumor cells were injected i.v. into B6-MAITcast MR1−/− mice. Tumor cell metastases were enumerated from lungs 14 days post tumor inoculation. Data is presented as mean ± SEM of n = 11 (non-treated) and n = 8 (5-OP-RU treated) independent mice from two combined experiments, two-tailed Mann–Whitney test. f Following treatment of mice as per (a). 5 × 105 E0771 tumor cells were injected i.v. into B6-MAITcast MR1 WT mice. Tumor cell metastases were enumerated from lungs 14 days post tumor inoculation. Data is presented as mean ± SEM of n = 13 (non-treated) and n = 14 (5-OP-RU treated) independent mice from two combined experiments, two-tailed Mann–Whitney test. g Schematic of experimental setup for therapeutic model. h 5 × 105 E0771 tumor cells were injected i.v. into B6-MAITcast MR1 WT mice on day 0. Mice received 50 µL of 233 μM 5-OP-RU or PBS on days 2, 3, 4, and 6 and tumor cell metastases were enumerated from lungs 14 days post tumor inoculation. Data is presented as mean ± SEM of n = 6 (non-treated) and 7 (5-OP-RU treated) independent mice and is representative of two independent experiments, two-tailed Mann–Whitney test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns = non-significant.
