Fig. 3: SARS-CoV-2 N protein interacts with NLRP3 protein.
From: SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation
HEK293T cells (a) or A549 cells (b) were co-transfected with HA-SARS-CoV-2-N and Flag-ctrl, Flag-NLRP3, Flag-pro-Caspase-1, or Flag-ASC. Cell lysates were immunoprecipitated using anti-Flag antibody and analyzed using anti-Flag and anti-HA antibody. Cell lysates (40 μg) was used as Input. HEK293T cells (c) or A549 cells (d) were co-transfected with Flag-NLRP3 and HA-SARS-CoV-2-N. Cell lysates were immunoprecipitated using anti-Flag antibody or anti-HA antibody, and analyzed using anti-Flag and anti-HA antibody. Cell lysates (40 μg) was used as Input. e Purified His-CT (10 μg) or His-N (10 μg) was incubated with cell lysates of Flag-NLRP3-transfected HEK293T cells. Cell extracts were incubated with Ni-NTA Agarose beads. Mixtures were analyzed by immunoblotting using anti-NLRP3 or anti-His antibody. Untreated protein includes His-SARS-CoV-2-N (1 μg), His-CT (1 μg), or HEK293T cell lysates were analyzed by immunoblotting (as input). HEK293T cells (f, h) or A549 cells (g, i) were transfected with Flag-SARS-CoV-2-N and HA-NLRP3 or HA-ASC for 24 h. Nucleus marker DAPI (blue), Flag-SARS-CoV-2-N (green), and HA-NLRP3 or HA-ASC (red) were then visualized with confocal microscopy. Flag-Ctrl, Flag-NC, or HA-NC means pcDNA3.1(+)–3× flag or pCAGGS-HA empty plasmid. Data were representative of three independent experiments. Source data are provided as a Source Data file.
