Table 2 Apparent kinetic parameters for GOase variants with HMF.

From: Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering

Variant Name

kcat,app (s−1)

KM,app (mM)

kcat/KM (s−1 M−1)

M3–5

123.9 ± 1.4

3.3 ± 0.1

38000 ± 1000

M4

132.7 ± 2.6

2.1 ± 0.2

63000 ± 6000

M5-1

153.9 ± 4.2

2.0 ± 0.2

77000 ± 8000

M5-2

139.0 ± 2.4

1.8 ± 0.1

77000 ± 4000

M6-A

200.5 ± 13.7

10.6 ± 2.8

19000 ± 5000

M6-B

115.4 ± 6.6

15.8 ± 2.9

7300 ± 1400

M7-1A

246.2 ± 10.7

15.3 ± 2.2

16000 ± 2000

M7-2A

204.6 ± 10.7

14.5 ± 2.6

14000 ± 3000

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