Fig. 3: Difference map analysis of the nucleotide occupancy in each of the eight conformations. | Nature Communications

Fig. 3: Difference map analysis of the nucleotide occupancy in each of the eight conformations.

From: CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin

Fig. 3

Model for each conformation is generated by rigid body fitting and followed by real space refinement. The panels show the nucleotide binding pocket of each conformation. The density (in green mesh) represents difference density attributable to nucleotide in the binding pocket. A model of ADP is shown here to illustrate the orientation of the nucleotide in the binding pocket. The difference map is displayed at +4σ.

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