Fig. 1: Discovery of a CDH12+ tumor cell population by single-nucleus sequencing. | Nature Communications

Fig. 1: Discovery of a CDH12+ tumor cell population by single-nucleus sequencing.

From: An N-Cadherin 2 expressing epithelial cell subpopulation predicts response to surgery, chemotherapy and immunotherapy in bladder cancer

Fig. 1

a Workflow for single nucleus sequencing; MIBC—muscle invasive bladder cancer. b UMAP of all nuclei (71,832) in MIBC dataset colored by unsupervised clustering. c Average gene expression per patient of marker genes for each cell type in b. d UMAP of all epithelial nuclei (52,983) in MIBC dataset colored by epithelial population. e Gene signature scores for published MIBC subtype gene sets. f Uroepithelial differentiation-related marker gene expression in each epithelial population, where the dot size indicates the percent of cells within the subtype with non-zero expression of the respective gene. g Gene-gene correlations partitioned into co-expression modules annotated for epithelial population enrichment. Gene ontology (GO) annotations are included with g:SCS multiple testing corrected p-values for hypergeometric testing. h Activity scores for SCENIC transcription factor regulons in each epithelial population. i Gene signature scores for stem-cell and neuroendocrine differentiation gene sets.

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