Fig. 6: Deletion of RSPO3 in osteoblasts decreased LRP6 and E3 ligases important for WNT canonical signaling.
From: RSPO3 is important for trabecular bone and fracture risk in mice and humans
a, b LRP6 protein analysis in primary calvarial osteoblasts isolated from CAGGCre-ER-Rspo3flox/flox mice cultured in osteogenic media for 1 day with or without prior Rspo3 inactivation using tamoxifen (TAM). Lane view from the software in the capillary-based electrophoresis immunodetection system (a) and protein levels (b) normalized to total protein. c, d pLRP6 protein analysis in primary calvarial osteoblasts isolated from CAGGCre-ER-Rspo3flox/flox mice cultured in osteogenic media for 1 day with or without prior Rspo3 inactivation using tamoxifen (TAM). Lane view from the software in the capillary-based electrophoresis immunodetection system (c) and protein levels (d) normalized to total protein. e–h mRNA expression analyses of Znrf3 (e), Rnf43 (f), Tcf7 (g) and Lef1 (h) in primary calvarial osteoblasts isolated from CAGGCre-ER-Rspo3flox/flox mice cultured in osteogenic media for 6 days with or without prior Rspo3 inactivation using TAM. Veh=vehicle. Individual values are presented in all graphs with the mean presented as horizontal lines and ±SEM as vertical lines. n = 4 wells per group. Experiments were repeated one (e, f), two (a, b, g, h) or at least three (c, d) times. Statistical analyses were performed using two-sided Student’s t test. Source data are provided as a Source Data file.
