Fig. 1: Reproductive 21 nt phasiRNAs triggered by miRNA miR11308 are abundant in wild strawberry.

a Expression of 21 nt reproductive phasiRNAs in different tissues and anther development stages. The key at the right indicates the abundance in units of log2(RP20M). RP20M: reads per 20 million mapped reads. Athst: anther stage. b Abundance of miR11308 members in log10(RP20M) in different tissues of wild strawberry. c Above: sequence logo denoting conservation of target site of miR11308 for 25 21-PHAS loci. Below: nucleotide sequence alignment of 21-PHAS loci with sequence similarity denoted by the intensity of the blue color showing that the miR11308 target site is the only conserved region for all the precursors. d Alignment of members of the miR11308 family in wild strawberry. The degree of conservation is denoted by intensity of the blue color; the consensus sequence of the alignment is shown with a sequence logo. e Abundance (RP15M) of small RNAs in both strands of an example locus padded with 500 base pairs, each side. sRNA sizes are denoted by colors, as indicated at the top. f Phasing score of same locus as e; the red dot indicates the highest phased sRNA position. The red dot also represents the coordinate (3,446,776), which exactly coincides with the predicted cleavage site of miR11308, denoted by the red arrow. Source data underlying Fig. 1a, b are provided as a Source Data file.