Fig. 5: Dynamic metabolite and protein responses to induction of the glycerol pathway. | Nature Communications

Fig. 5: Dynamic metabolite and protein responses to induction of the glycerol pathway.

From: Metabolome and proteome analyses reveal transcriptional misregulation in glycolysis of engineered E. coli

Fig. 5

a Growth of the base strain (black) and the pBAD-Cra strain (orange). Glycerol production was induced by supplementing 0.5% ara to the medium at t = 0 h. Small dots show n = 2 independent shake flasks, and large dots are the mean. b Specific glycerol production rates (mmol g−1 h−1) were calculated for two adjacent time points as Δcgly/cx/Δt, where Δt is the time interval between the two time points; Δcgly is the difference of glycerol in the medium; cx is the biomass concentration. c Time-course of GPD1 levels and phosphoenolpyruvate synthetase (PpsA) levels. Proteomics samples were collected from the base strain (black) and the pBAD-Cra strain (orange) (a). Data are normalized to the 0 h time point of the base strain. d Time-course of the concentration of intracellular metabolites (Hexose-P hexose-phosphates, FBP fructose-1,6-bisphosphate, DHAP dihydroxyacetone phosphate, PEP phosphoenolpyruvic acid). Metabolomics samples were collected from the base strain (black) and the pBAD-Cra strain (orange) (a). Data are normalized to the 0 h time point of the base strain. Note that in b–d, the small dots show samples from n = 2 independent shake flasks and big dots are the mean. Source data are provided in the Source Data file.

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