Fig. 4: MOB1 knockdown protects from β-cell apoptosis in vitro. | Nature Communications

Fig. 4: MOB1 knockdown protects from β-cell apoptosis in vitro.

From: The Hippo kinase LATS2 impairs pancreatic β-cell survival in diabetes through the mTORC1-autophagy axis

Fig. 4

a, b Representative western blots and pooled quantitative densitometry analysis (lower panels) of INS-1E cells transfected with siMOB1 or control siScr and treated with a 22.2 mM glucose or b 22.2 mM glucose plus 0.5 mM palmitate for 48 h (n = 4 independent experiments; n = 3 for clPARP). c Representative western blot and pooled quantitative densitometry analysis (lower panel) of INS-1E cells transfected with siMOB1 or siScr and transduced with Ad-LacZ or Ad-LATS2 for 48 h (n = 3 independent experiments). Results were normalized to the respective control conditions and ratios, in which a normal distribution of results cannot be proven, were analyzed. Data are expressed as means ± SEM. *p < 0.05, **p < 0.01; all by two-tailed Student’s t-tests.

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