Fig. 3: Aberrant synthesis of mitochondrial components and alterations in phosphorylation of fission proteins changes mitochondrial morphology and function.
From: The pathogenesis of mesothelioma is driven by a dysregulated translatome
a Representative electron micrographs of primary MpM cell lines (Meso 7 T, Meso 8 T, Meso17T) and control cells (NMS). Scale bar 1 μm. b The average number of enlarged particles per field. In all, 19–23 different micrographs (for n = 369 mitochondrial particles) were analysed per sample. Error bars represent SD and significance was assessed using Mann–Whitney test, p values are shown on the bar graphs. The average number of elongated particles per field. In all, 19–23 different micrographs (n = 369 mitochondrial particles) were analysed per sample. Error bars represent SEM and the measure of the centre of the error bars is the mean, significance was assessed using Mann–Whitney test p values are shown above the bars. c Confocal images of primary MpM cells or healthy mesothelial control, stained with DAPI (blue-nuclei) and ATPB (red-mitochondria). Scale bar 20 μm or 4 μM for the call outs. d The mitochondrial networks in the samples from c were assessed in terms of the extent of elongated, mixed or enlarged/fragmented mitochondria and the data represented as pie charts, (black sections = elongated, light grey sections = mixed and dark grey = enlarged). The significance of changes in the elongated mitochondrial network was assessed using two-sided unpaired Student’s t test NM vs tumour cell lines. P values are shown above the pie charts. e Western blot analysis of eight primary cell lines derived from patients with MpM and from untransformed mesothelium (NM), which were probed with antibodies against phospho-Ser616 DRP, DRP1, MTPF1 and Mitofusin2. Beta-actin was used as a sample integrity control. Source data are provided as a Source Data file. f Densitometric analysis of the western blots in data shown in Fig. 2e. Protein levels in MpM-derived cell lines are compared to NM control cells. Error bars represent standard deviation, and the measure of the centre of the error bars is the mean (n = 8 biological repeats). Significance was assessed using a two-sided unpaired student’s t test with multiple comparisons, p values are shown on the bar graphs.
