Fig. 7: mTORC1 and 2 inactivation inhibits tumour cell growth ex vivo and in mouse models of MpM.
From: The pathogenesis of mesothelioma is driven by a dysregulated translatome
a Representative sections of mesothelioma explants stained with the indicated antibody. Cytokeratin staining was used to identify tumour areas within the explants. Scale bar 1 μm, 200 μm for the call outs. b Mesothelioma explants were cultured for 72 h with 100 nM AZD2014 or with vehicle. H-score quantification of IHC staining within tumour areas is performed by Visiopharm® software. Mann–Witney significance test was performed (two patients, n = 8 explants, each p values are shown on the graph). The box plots show the box from the first quartile to the third quartile. The middle line represents the median. The whiskers go up to the maximum and minimum values. c Adult mice (Cdkn2a−/−;Nf2fl/fl;Tp53fl/fl) were intrapleurally injected with 107 pfu Lenti-Cre, followed after 10 days by a single intrapleural injection of 25 μg of long fibre amosite asbestos and allowed to age for a further 50 days. The mice were treated with AZD2014 + AZD8186 (n = 9 mice) or vehicle control (n = 9 mice), from d60 post-viral induction of floxed alleles. Graph shows survival from the first day of treatment (Mantel–Cox log-rank test).
