Fig. 2: PERM1 is specifically enriched in SSM, and SSM are altered in Perm1−/− muscles.
From: PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B
a, b Volcano plot (a) and boxplot (b) depicting the regulation of PERM1, MitoCarta-annotated, OMM, and MICOS–MIB proteins in isolated IFM and SSM from wild-type TA muscle. Mitochondrial and OMM proteins are enriched in IFM. Inversely, PERM1 is clearly more abundant in the SSM fraction (n = 3 mice per group). c, d Comparison of log2 differences in MitoCarta 2.0 proteins compared to the complete dataset revealed no changes in the overall regulation of mitochondrial proteins in (c) isolated IFM of Perm1−/− TA muscle compared to wild-type control, though MitoCarta-annotated proteins were significantly reduced in (d) isolated SSM from Perm1−/− TA muscle compared to wild-type control (n = 3 mice per group). e, f) Volcano plots highlighting the regulation of sarcomeric proteins and proteins of the MICOS–MIB complex in (e) isolated IFM from Perm1−/− TA compared to wild-type control and in (f) isolated SSM from Perm1−/− TA compared to wild-type control (n = 3 mice per group). Box plots (b, c, d, e) represent the median, 25th, and 75th percentiles, maximum and minimum are connected through whiskers. Outliers are defined as Q1–1.8 IQR and Q3+1.8 IQR. b–d unpaired two-sided Mann-Whitney U-test. Source data are provided in Supplementary Data 1.
