Fig. 6: PERM1 associates with ankyrin B and the interaction with the MICOS–MIB complex is dependent on the transmembrane helix.
From: PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B
a PERM1 interacts with ANKB, as indicated by immunoprecipitation of FLAG-tagged Perm1 and HA-tagged ankyrin-B in TREx-293 cells stably expressing Perm1-FLAG. Protein lysates were immunoprecipitated with anti-FLAG beads and anti-HA beads and immunoblotted with anti-FLAG and anti-HA antibodies. b Immunohistochemical staining of soleus muscle revealed co-localization of PERM1 (red) with ANKB (green). Scale, 50 µm. c Mean fractional intensity of ANKB signals in bins 1–6 from Perm1−/− and wild-type soleus muscle fibers. ANKB staining intensity was reduced at subsarcolemmal sites and increased within interfibrillar mitochondria in Perm1−/− muscles compared to wild-type controls (n = 4 mice per group, 2299 quantified fibers). Box plot (c) represents the median, 25th, and 75th percentiles, maximum and minimum are connected through whiskers. Outliers are defined as Q1–1.8 IQR and Q3+1.8 IQR. (c) unpaired two-sided Mann-Whitney U-test. Source data are provided as Source Data file.
