Fig. 2: Immunofluorescent staining and gene regulatory networks in wild-type in vitro cultured NHP embryos.
From: Amnion signals are essential for mesoderm formation in primates
See also Supplementary Fig. 6 and Supplementary Data 4. a, b immunofluorescent staining of major cell types at Day 14 identified in the scRNA sequencing. n = 2 wild-type embryos a OCT4, VIM, GATA3, TFAP2C; b ISL1, GABRP, MIXL1, BRA, and SOX17. Scale bar = 50 µm. White numbers indicate section numbers and arrows indicate the signal of interest. c Binary activity matrix of regulons identified at Day 14 by gene regulatory network inference active in at least 1% of the cells clustered unsupervised. Selected master regulators are depicted in the color corresponding to the cell type they show activity in. d Binarized gene set activity of the selected regulons at Day 14 in the different cell types depicted on the UMAP plot from Fig. 1d. Epi epiblast, Endo endoderm, ExE-Mech extraembryonic mesenchyme, AM-1 amnion 1, AM-2 amnion 2, meso-1 mesoderm 1, meso-2 mesoderm 2, D dorsal, V ventral, A anterior, P posterior.
