Fig. 6: eIF6 depletion preserves mitochondrial bioenergetic capacity. | Nature Communications

Fig. 6: eIF6 depletion preserves mitochondrial bioenergetic capacity.

From: Targeting of eIF6-driven translation induces a metabolic rewiring that reduces NAFLD and the consequent evolution to hepatocellular carcinoma

Fig. 6

a Gene ontology (GO) analysis identifies upregulated mitochondrial pathways in eIF6+/− livers. b Scheme showing the primary hepatocytes isolation outline. c Representative western blotting of eIF6 and FASN in primary hepatocytes, isolated from HFD-fed mice. d Left: bright-field images of primary hepatocytes isolated from eIF6+/− and eIF6+/+ obese mice. eIF6+/+ hepatocytes have an increased accumulation of lipid droplets. Scale bars are indicated. Right: quantification of Oil Red O staining in primary hepatocytes. Data are represented as means ± SD, n = 3 for genotype. Two-tailed t test. e Left: immunofluorescence of primary hepatocytes stained for Tomm20 (red) and DAPI (blue). Autofluorescence (green) was used to visualize cells. Scale bars are indicated. Right: quantification of Tomm20 fluorescence intensity levels shows that they are increased in eIF6+/− hepatocytes. Mann–Whitney test. f, g eIF6+/− primary hepatocytes have increased ATP levels (f) but similar lactate secretion (g). Two-tailed t test. Data are represented as means ± SD of independent samples. Data are provided as a Source Data File.

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