Fig. 1: Schematic overview of the hRUV pipeline.
From: A hierarchical approach to removal of unwanted variation for large-scale metabolomics data
a A schematic illustration of the plasma sample arrangement for the experimental batch in an array format where intra-batch sample replicates (green circles) and inter-batch sample replicates (blue circles) are embedded. b A schematic illustration of overall sample replicate design and arrangements. c Continuing the colour scheme from b, two illustrative run plots requiring intra-batch correction, with signal drift and other variations illustrated in the grey boxes. d A demonstration of signal variation before and after inter-batch correction in hRUV. A common approach to inter-batch correction is illustrated as conventional RUV and the proposed hierarchical approaches are illustrated. e A list of evaluation criteria to assess hRUV performances grouped into categories of biological signals, variability and reproducibility and distributional characteristics. f A screenshot of the user-friendly shiny application.
