Fig. 2: Elmo1-deficient mice display reduced bone erosion in two models of arthritis.
From: ELMO1 signaling is a promoter of osteoclast function and bone loss
a Schematic of collagen-induced arthritis (CIA) induction in Elmo−/−-DBA/1 J mice. b Expression of indicated genes was analyzed in the total paw extracts from either healthy Elmo1+/+-DBA (WT Ctrl, black, n = 2), or Elmo1+/+-DBA (WT, black, n = 6) and Elmo−/−-DBA (KO, green, n = 6) mice on day 50 of CIA by quantitative RT-PCR. Each symbol represents a mouse. Data are presented in a Box and Whiskers format with the box indicating 25th–75th percentile, all data points shown and median indicated. ***p < 0.001, ****p < 0.0001, Student’s t test, two-tailed, unpaired. c MicroCT images of mouse ankles at the chronic phase of CIA. The significant bone remodeling in Elmo1+/+-DBA mice (wild type), indicated by yellow brackets, is absent in Elmo1 KO. The yellow arrow denotes bone erosion in the calcaneus. One representative of three mice analyzed is shown. Scale bar = 1 mm. d Bone erosions were quantified in the area of the calcaneus of Elmo1+/+-DBA (WT, black, n = 3) and Elmo−/−-DBA (KO, green, n = 3) mice in the chronic phase of CIA, as described in the Methods. Data are presented in a Box and Whiskers format with the box indicating 25th–75th percentile, all data points shown, and median indicated. **p < 0.01, Student’s t test, two-tailed, unpaired. e Schematic of the K/BxN serum transfer mediated arthritis induction in Elmo−/− C57BL/6 mice. f Expression of indicated genes was analyzed in the total paw extracts from Elmo1+/− (black, n = 7) and Elmo1−/− (green, n = 7) mice on day 10 after K/BxN serum injection. Each symbol represents a mouse. Data are presented in a Box and Whiskers format with the box indicating 25th–75th percentile, all data points shown, and median indicated. *p < 0.05, **p < 0.01, Student’s t test, two-tailed, unpaired. g Bone erosions were quantified in the H&E-stained histology sections of mouse ankles from Elmo1+/− (black, n = 7) and Elmo1−/− (green, n = 7) mice on day 10 of K/BxN serum-induced arthritis. Each symbol represents a mouse. Data are presented in a Box and Whiskers format with the box indicating 25th–75th percentile, all data points shown, and median indicated. ***p < 0.001, Student’s t test, two-tailed, unpaired. h Osteoclasts were differentiated from the bone marrow of RosaYFP and RosaYFP-Cx3cr1-Cre mice and analyzed (day 6) for YFP protein expression by microscopy. Scale bar, 0.05 mm. A representative of two independent experiments is shown. i Confirmation of loss of ELMO1 expression in osteoclasts from Cx3cr1-Cre-Elmo1fl/fl mice. ELMO1 protein expression in whole cell extracts from macrophages or osteoclasts differentiated from the bone marrow of indicated mice. ERK was used as a protein loading control. A representative of two independent experiments is shown. j Schematic of the K/BxN serum transfer mediated arthritis induction in Elmofl/flCx3cr1-Cre mice. k Bone erosions were quantified in the H&E-stained histology sections of female Elmo1fl/flCx3cr1-Cre (green, n = 8) and Elmo1fl/fl (black, n = 6) on day 10 of K/BxN serum-induced arthritis. Each symbol represents a mouse. Data are presented in a Box and Whiskers format with box indicating 25th–75th percentile, all data points shown, and median indicated. *p < 0.05, Student’s t test, two-tailed, unpaired. Source data are provided as the Source data file.
