Fig. 4: Effect of 2PS gene copy numbers and substrates on TAL production. | Nature Communications

Fig. 4: Effect of 2PS gene copy numbers and substrates on TAL production.

From: Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast

Fig. 4

a Improved production of TAL by increasing the copy numbers of 2PS genes (***P < 0.001 Tal1 xylose vs glucose, ***P < 0.001 Tal2 xylose vs glucose, ***P < 0.001 Tal3 xylose vs glucose, ***P = 0.002 Tal4 xylose vs glucose, ***P < 0.001 Tal2 vs Tal1 in xylose, *P = 0.014 Tal3 vs Tal2 in xylose, ***P < 0.001 Tal4 vs Tal3 in xylose). b Yield of TAL from different single and mixed substrates by the Tal4 strain. Calculated from cultures of Tal4 strain with 10 g/L ethanol, 10 g/L acetate, 40 g/L glucose, 40 g/L xylose, 40 g/L glucose + 10 g/L acetate and 40 g/L xylose + 10 g/L acetate, respectively (TAL Yield: ***P < 0.001 Xylose + Acetate vs Glucose + Acetate, ***P < 0.001 Xylose + Acetate vs Glucose, ***P < 0.001 Xylose + Acetate vs Ethanol, ***P < 0.001 Xylose + Acetate vs xylose). c Production of TAL by the Tal4 strain from 40 g/L xylose with supplementation at varying amounts of acetate (***P < 0.001 5 vs 0 g/L, ***P = 0.001 10 vs 5 g/L, *P = 0.025 10 vs 15 g/L, ***P < 0.001 10 vs 20 g/L). Data are presented as mean value and standard deviations of three independent biological replicates. Statistical significance of the differences was evaluated by one-way ANOVA followed by Tukey’s multiple-comparison test. Source data are provided as a Source Data file.

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