Fig. 3: A hybridization intermediate for \({{{{{\mathrm{dsGGACU}}}}}}^{{{{{{\mathrm{m}}}}}}^6{{{{{\mathrm{A}}}}}}6}\) at T = 55 °C. | Nature Communications

Fig. 3: A hybridization intermediate for \({{{{{\mathrm{dsGGACU}}}}}}^{{{{{{\mathrm{m}}}}}}^6{{{{{\mathrm{A}}}}}}6}\) at T = 55 °C.

From: A quantitative model predicts how m6A reshapes the kinetic landscape of nucleic acid hybridization and conformational transitions

Fig. 3

a 13C CEST profile for m6A6 C2 in \({{{{{\mathrm{dsGGACU}}}}}}^{{{{{{\mathrm{m}}}}}}^6{{{{{\mathrm{A}}}}}}6}\) at T = 55 °C shows a second dip at ΔωES that is distinct from the ssRNA ES at Δωss. b Exchange parameters (Supplementary Table 3) from a three-state fit to the RD data using a triangular model. c Zoom in to the m6A6 C2 CEST profiles comparing results from an unconstrained three-state fit to the Bloch–McConnell equations assuming the triangular model and a constrained three-state fit assuming a linear CS model. Data for CEST profiles were presented as mean values ± 1 s.d. (smaller than data points) from n = 3 independent measurements of peak intensities at zero relaxation delay (see “Methods”).

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