Fig. 5: Testing a four-state CS + IF kinetic model. | Nature Communications

Fig. 5: Testing a four-state CS + IF kinetic model.

From: A quantitative model predicts how m6A reshapes the kinetic landscape of nucleic acid hybridization and conformational transitions

Fig. 5

a Schematic of the CS + IF model with populations and kinetic rate constants measured at T = 55 °C for \({{{{{\mathrm{dsGGACU}}}}}}^{{{{{{\mathrm{m}}}}}}^6{{{{{\mathrm{A}}}}}}}\). b Constrained four-state (CS + IF model) shared fit (solid lines) of the m6A C2 and C8 13C CEST profiles to the Bloch–McConnell equations for \({{{{{\mathrm{dsGGACU}}}}}}^{{{{{{\mathrm{m}}}}}}^6{{{{{\mathrm{A}}}}}}}\) at T = 55 and 65 °C. \({\chi }_{{{{{{\mathrm{red}}}}}}}^{2}\) values were obtained from global fitting m6A-C2 and m6A-C8 CEST data. RF field powers for CEST profiles are color-coded. Data for CEST profiles in panel (b) were presented as mean value ± 1 s.d. (smaller than data points) from n = 3 independent measurements of peak intensities at zero relaxation delay (see “Methods” section). c Equilibrium flux through CS and IF pathways at T = 55 and 65 °C.

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