Fig. 6: Heart organoids recapitulate functional and structural features of the developing heart. | Nature Communications

Fig. 6: Heart organoids recapitulate functional and structural features of the developing heart.

From: Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease

Fig. 6

a Optical coherence tomography images showing cross-sections through an organoid, revealing chambers; scale bar: 500 µm. b TEM images of hHOs showing endoplasmic reticulum (ER), gap junctions (Gj), glycogen granules (Gy), lipid droplets (Ld), mitochondria (Mi), nucleus (N), and sarcomeres (S); scale bars: 2 µm (top), 1 µm (bottom). c Immunofluorescence images of myocardial tissue in hHOs showing WGA staining of T-tubule-like structures (green); white arrowheads indicate representative T-tubule-like structures between cardiomyocytes; scale bar: 50 µm, inset: 20 µm. d Electrophysiology recordings of 4 organoids on microelectrode array spanning 15 s and a representative action potential wave (inset). e Ca2 + transients in 4 representative hHOs after two weeks of differentiation.

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