Fig. 1: Characterization of hACE2-blocking MAb h11B11.
From: A broadly neutralizing humanized ACE2-targeting antibody against SARS-CoV-2 variants
a h11B11 blocks SARS-CoV-RBD and SARS-CoV-2-RBD binding to hACE2. The quantified data were shown in the right panel. Mean values of three biological replicates ± SEM (standard error of the mean) are shown (unpaired t test, two tailed). Data are representative of three independent experiments. b, c The binding kinetics of h11B11 to wild-type and mutated hACE2 was assessed using a single-cycle model. The kinetic parameters were labeled accordingly. Values represent mean ± SEM of three independent assessments. n.d. means the values are not detectable due to poor binding ability. d Measurement of enzymatic kinetic constants of hACE2 in the presence of h11B11 and control IgG protein. Actual data points and Michaelis–Menten plots for hACE2 hydrolysis of Mca-AFK-Dnp in the absence (purple) or presence (orange, green, and blue) of h11B11 protein. Isotype IgG (yellow) and inhibitor (black) were used as control. The initial velocity conditions were limited to 12 min. The value in the x-axis of d represents the concentrations of substrate (Mca-AFK-Dnp).
