Fig. 4: The safety of h11B11 in vitro and in cynomolgus monkeys.

a HEK293-hACE2 cells were incubated with 10 μg/ml h11B11 antibody for the indicated times, followed by western blot analysis of indicated proteins. b HEK293-hACE2 cells were incubated with 10 μg/ml h11B11 antibody for the indicated times, followed by membrane–cytoplasm fractionation analysis of indicated proteins. The antibody used for western blot in a, b was anti-ACE2 pAb. Data are one representative result of two independent experiments. c HEK293-hACE2 cells were incubated with serially diluted h11B11 antibody or anti-IgG control antibody. After incubation at 37 °C for 4 h (c) or 24 h (d), the hACE2–antibody complex was detected by flow cytometry. Mean values of three biological replicates mean ± SEM (standard error of the mean) are shown. Data are representative of three independent experiments. e, f The blood pressure of cynomolgus monkeys in low and high groups after the second dosing. 1001, 1002, 1101, and 1102 represent animal numbers. SP is the systolic pressure and DP is the diastolic pressure. Mean is the average of SP and DP. The value was tested once. g, h The hematology and serum chemistry (toxicology) assays of four animals for 3 weeks. Cynomolgus monkeys were injected 60 or 180 mg/kg h11B11 once a week for three times. WBC white blood cell, RBC red blood cell, MONO monocyte, LYMPH lymphocyte, AST aspartate transaminase, ALT alanine transaminase, TBIL total bilirubin.