Fig. 4: Structural basis for flexible 3′ seed region in TtCmr complexes. | Nature Communications

Fig. 4: Structural basis for flexible 3′ seed region in TtCmr complexes.

From: SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation

Fig. 4

a Overall structure of TtCmr complex with 46 nt crRNA (EMD-2898). b Overall structure of TtCmr complex with a 40 nt crRNA (EMD-2899). Complexes are colored as in Fig. 1. Boxed regions refer to close-up views shown in panels c and d. c Close-up view of the top of TtCmr46. d Close-up view of the top of TtCmr34. The ‘open’ seed region located at the 3′ end of the crRNA is more accessible than the upstream regions located towards the 5′ end, which are protected by Cmr5 subunits. The 3′ end of the crRNA is thus primed for propagating conformational changes and repositioning Cmr5 subunits along the complex upon target binding.

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