Fig. 6: Schematic model of TtCmr target binding and subsequent activities. | Nature Communications

Fig. 6: Schematic model of TtCmr target binding and subsequent activities.

From: SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation

Fig. 6

(1) TtCmr complex with bound crRNA is scanning for complimentary target RNA. (2) Target RNA binding is initiated at seed region, which induces a conformational change that allows further base pairing. (3) Full base pairing of target RNA to the crRNA, activating Cas10. (4) Target RNA is cleaved by Cas7 subunits and cOA is produced by Cas10. (5) Cleaved target RNA dissociates from TtCmr.

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