Fig. 2: Chronic exposure to 27HC selects for cells with increased malignant phenotypes. | Nature Communications

Fig. 2: Chronic exposure to 27HC selects for cells with increased malignant phenotypes.

From: Dysregulated cholesterol homeostasis results in resistance to ferroptosis increasing tumorigenicity and metastasis in cancer

Fig. 2

a ER-negative cancer cells were treated with 0.1% DMSO or 27HC (5 µM) for 1–4 months. Emergent cells are resistant to the antiproliferative effects of 27HC. Representative data from 4T1 (left), Py230 (middle), and BPD6 (right) cancer models are shown. Data plotted as mean ± SEM as representative results from three independent experiments, n = 5 wells of cells. b Xenograft tumors from 27HCS and 27HCR cells in 4T1 (upper), Py230 (middle), and BPD6 (lower) in syngeneic mouse models. (4T1-27HCS, n = 8 mice; 4T1-27HCR, n = 8 mice; Py230-27HCS, n = 6 mice; Py230-27HCR, n = 8 mice; BPD6-27HCS, n = 8 mice; BPD6-27HCR, n = 8 mice), Data plotted are mean ± SEM, P values were calculated using two-way ANOVA. c Lung metastasis from 27HCS- and 27HCR cells in 4T1 (upper), Py230 (middle), and BPD6 (lower) intravenously injected in syngeneic mouse models. Mice were euthanized on Day 21 (4T1), Day 78 (Py230), and Day 20 (BPD6), respectively. Numerical source data are reported in the Source Data File.

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