Fig. 3: Increased lipid uptake is a feature of cells resistant to the antiproliferative actions of 27HC.

a qRT-PCR profiling of the expression of select genes involved in lipid metabolism in 27HCS and 27HCR cells treated with 0.1% DMSO or 27HC (5 µM) for 24–72 h. Representative data from 4T1, Py230, and HCC1954 cancer models are shown. Data are plotted as mean ± SEM as representative results from two (HCC1954), four (Py230), and one (4T1) independent experiments, n = 4 wells of cells. b Lipid droplet content in 27HCS and 27HCR derivitives of 4T1, Py230, and HCC1954 cells were visualized using BODIPY 493/503 staining. Data are plotted as mean ± SEM as representative results from three independent experiments, n = 5 wells of cells c 27HCS and 27HCR derivatives of 4T1, Py230, and HCC1954 cells were cultured in lipid-rich (FBS) and lipid-depleted (DL-FBS) serum-containing media and treated with 0.1% DMSO or 27HC at indicated doses, followed by assessment of cell growth. Data are plotted as mean ± SEM as representative results from three independent experiments, n = 5 wells of cells d Migration assays performed using 27HCSand 27HCR derivatives of 4T1, Py230, and HCC1954 cells cultured in lipid-rich (FBS) and lipid-depleted (DL-FBS) serum-containing media. Data are plotted as mean ± SEM; n = 8-20 random fields measurements from a total of three transwell chambers. e qRT-PCR analysis of the mRNA expression levels of genes involved in lipid uptake and trafficking in 27HCS and 27HCR derivatives of 4T1, Py230, and HCC1954 cells. Representative results from Py230 cells are shown, and results for other cell lines are shown in Supplementary Data 4. Data are plotted as mean ± SEM as representative results from two (4T1 and HCC1954) and eight (Py230) independent experiments; n = 4 wells of cells; P values were calculated using a two-sided unpaired Student’s t test a, b, and e and one-way ANOVA with Tukey’s post hoc test c and d. Numerical source data are reported in the Supplementary Data 2 and in the Source Data File.