Fig. 2: Impaired burst firing in Shank2–/– mPFC inhibitory neurons. | Nature Communications

Fig. 2: Impaired burst firing in Shank2–/– mPFC inhibitory neurons.

From: Excitatory synapses and gap junctions cooperate to improve Pv neuronal burst firing and cortical social cognition in Shank2-mutant mice

Fig. 2

a Classification of mPFC neurons into putative excitatory and inhibitory neurons. Total neuronal firings, grouped into putative excitatory and inhibitory firings, based on peak-to-valley ratio and half-valley width. Total spikes from WT (black) and Shank2–/–(red) mice were plotted three-dimensionally against peak-to-valley ratio, half-valley width, and firing rate. Spikes were grouped into putative excitatory and inhibitory firings based on peak-to-valley ratio and half-valley width, as follows: excitatory firings, peak-to-valley ratio >1.5 and half-valley width >250 µs; inhibitory firings, peak-to-valley ratio <1.5, and half-valley width <250 µs. b Increased firing rates in pExc, but not pInh, neurons upon target encounters, as shown by mean-firing rate (±SEM) of pExc and pInh neurons of WT and Shank2–/– mice in the resting (R) state and with E/O/S encounters (n = 295 [WT] and 409 [KO], *p < 0.05, **p < 0.01, ***p < 0.001, Friedman test with Dunn’s multiple comparison; p = 0.0005 [R–E], p = 0.0005 [R–O], p = 0.0290 [R–S], p = 0.0072 [WT-pExc], p < 0.0001 [R–E], p = 0.0109 [R–O], p < 0.0001 [R–S], p = 0.0030 [KO-pExc], p = 0.258 [WT-pInh], p = 0.118 [KO-pInh]). c ISI histograms for two sample pInh neurons (left, from WT; right, from KO mouse) in the resting (R) state and with E/O/S encounters. The numbers in the parenthesis indicate single-neuron numbers. d Bar graphs for Δburst firing (changes in high-frequency burst firings [<12 ms of ISI] during S/O/E target encounter relative to the R state) for E, O, and S in WT and KO-pInh neurons (n = 36 [WT] and 41 [KO] for pInh, 286 [WT] and 340 [KO] for pExc, *p < 0.05, **p < 0.01, two-way repeated-measures/RM-ANOVA with Sidak’s multiple comparison test [bar graph]; genotype p = 0.0129, target p = 0.2808, interaction p = 0.0082 [pInh], E–R p = 0.1945, O–R p = 0.350, S–R p = 0.0011 [WT vs KO, pInh], E–R vs O–R p = 0.95, E–R vs S–R p = 0.032, O–R vs S–R p = 0.0085 [WT-pInh], E–R vs O–R p = 0.997, E–R vs S–R p = 0.762, O–R vs S–R p = 0.650 [KO-pInh], genotype p = 0.249, target p = 0.515, interaction p = 0.450 [pExc]). See Source data for raw data values and Supplementary Table 1 for statistical details.

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