Fig. 5: Burst firing in Shank2–/– Pv neurons strongly induced by 10-Hz, but not 40-Hz, stimulation. | Nature Communications

Fig. 5: Burst firing in Shank2–/– Pv neurons strongly induced by 10-Hz, but not 40-Hz, stimulation.

From: Excitatory synapses and gap junctions cooperate to improve Pv neuronal burst firing and cortical social cognition in Shank2-mutant mice

Fig. 5

a Increased burst firings in single Pv neurons focally light-stimulated in the cell body in the Shank2–/– mPFC (prelimbic, layer 2/3), as shown by proportions of neurons with specific firing patterns (no-fire, tonic-fire, burst-fire). Note that the mean-firing rate shows only an increasing tendency. A single Pv neuron in acute slices from Pv-Cre;Shank2–/– or Pv-Cre;WT (control) mice (>12 weeks) infected in the mPFC with AAV-DIO-ChR2-EYFP (3 weeks) was stimulated by blue light (473 nm, 10 Hz, 5 ms, 12 epochs) (n = 42 neurons from 9 mice [WT], 31/12 [KO], **p < 0.01, ns, not significant, Chi-square test [proportion], two-tailed Mann–Whitney test [firing rate]; p = 0.0028 [proportion], p = 0.2561 [firing rate]). b Increased burst firings in Pv neurons neighboring a light-stimulated single Pv neuron in the Shank2–/– mPFC, as shown by proportions of firing patterns and mean-firing rates (n = 47/10 [WT], 42/15 [KO], ***p < 0.001, Chi-square test [proportion], two-tailed Mann–Whitney test [firing rate]; p < 0.0001 [proportion], p < 0.0001 [firing rate]). c, d Limited burst firings in light-stimulated and neighboring Pv neurons by 40 Hz stimulation in the Shank2–/– mPFC (n = 32/8 [WT], 30/10 [KO] for light-stimulated Pv neurons, 37/9 [WT], 17/9 [KO] for neighboring Pv neurons, **p < 0.01, ***p < 0.001, ns, not significant, Chi-square test [proportion], two-tailed Mann–Whitney test [firing rate]; p = 0.7524 [proportion-c], p = 0.671 [firing rate-c], p < 0.0001 [proportion-d], p = 0.0067 [firing rate-d]). See Source data for raw data values and Supplementary Table 1 for statistical details.

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