Fig. 6: Gap junction-dependent burst firing in Shank2–/– Pv neurons.
a Computational model simulations demonstrate that the number and the strength of individual gap junctions of a Pv neuron are positively correlated with the extents of Pv neuronal burst firings, as monitored in light-stimulated single Pv neurons or in Pv neurons neighboring a light-stimulated single Pv neuron. Note that 40-Hz stimulation is less effective than 10-Hz stimulation for inducing burst firings, probably due to the low-pass filtering characteristics of gap junctions. b Mefloquine (25 µM) treatment decreases burst and tonic firings and firing rates in Shank2–/– Pv neurons neighboring a light-stimulated single Pv neuron, whereas it minimally affects burst firing in WT Pv neurons, although tonic firing and firing rate are decreased (prelimbic, layer 2/3, 12–13 weeks). Data: minimal, maximal, median, 25%, and 75% values (n = 40 neurons from 13 mice [KO-V/vehicle, before mefloquine/MQ treatment; KO-MQ/mefloquine, after MQ treatment] and 29 neurons from 13 mice [WT-V and WT-MQ], *p < 0.05, ***p < 0.001, ns, not significant, Chi-square test [proportion], two-way ANOVA with Sidak’s test [firing rate]; p < 0.0001 [WT-proportion], p < 0.0001 [KO-proportion], interaction p 0.0051, drug p < 0.0001, group p 0.8354 [firing rate], WT p = 0.0105, KO p < 0.0001 [Veh-Mef, firing rate]). See Source data for raw data values and Supplementary Table 1 for statistical details.
