Fig. 3: Overexpression of Sll1077 accelerates guanidine degradation and promotes biomass growth in Synechocystis 6803.
From: A guanidine-degrading enzyme controls genomic stability of ethylene-producing cyanobacteria
a Strategies for enhancing the overexpression of gene sll1077 in Synechocystis 6803. Gene sll1077 was overexpressed driven by the tac promoter, with its RBS (purple nucleotides, including the entire 5’-untranslated sequence upstream of the start codon of gene sll1077) at the 5’-region and the His tag and terminator at the 3’-region optimized. b SDS-PAGE and western blotting (His tag) showing the improved expression of Sll1077 in Synechocystis. Representative data from two independent experiments. c Guanidine-degradation profiles of Synechocystis 6803 and sll1077-overexpressing strains. d Cell growth curves for Synechocystis 6803 and sll1077-overexpressing strains, indicated by readings of OD730 of cell cultures. Data represent means and standard deviations from three biological replicates. Source data underlying panels (c, d) are provided as a Source Data file.
