Fig. 7: Chmp1a knockdown enhanced ferroptosis through iron accumulation. | Nature Communications

Fig. 7: Chmp1a knockdown enhanced ferroptosis through iron accumulation.

From: A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis

Fig. 7

a (left) Representative BODIPY 581/591 C11 staining of siControl and siChmp1a transfected cell following sham or cisplatin treatment. Scale bar: 50 μm. (right) Quantification of the oxidized vs. reduced probe (n = 5). b Relative mRNA level of Acsl4 in the kidneys of folic acid and cisplatin-treated wild-type and Chmp1a+/− mice. Sham-treated group: WT (n = 3), Chmp1a+/− (n = 3); FA-treated group: WT (n = 5), Chmp1a+/− (n = 5); cisplatin-treated group: WT (n = 4), Chmp1a+/− (n = 5). c Western blots of ACSL4 in kidneys of folic acid-treated wild-type and Chmp1a+/− mice. d Representative images of ACSL4 immunostaining of kidney sections from folic acid-treated wild-type and Chmp1a+/− mice. Scale bar: 10 μm. e LDH level of siControl and siChmp1a transfected cell with or without cisplatin and ferroptosis inhibitor liproxstatin1 (n = 3). f (upper) Western blots of CD63 of kidney exosomes of wild-type and Chmp1a+/− mice. (bottom) Quantification of three independent experiments (n = 3). g Ferrous iron concentrations (normalized to kidney weight) of kidneys of cisplatin-treated wild-type and Chmp1a+/− mice. Sham-treated group: WT (n = 3), Chmp1a+/− (n = 3); cisplatin-treated group: WT (n = 4), Chmp1a+/− (n = 5). h Serum creatinine level of control and Chmp1a+/− mice with or without cisplatin and liproxstatin injection. Sham-treated group: WT (n = 3), Chmp1a+/− (n = 3); cisplatin-treated group: WT (n = 5), Chmp1a+/− (n = 5); cisplatin and liproxstatin-treated group: WT (n = 4), Chmp1a+/− (n = 4). i Relative transcript level of injury marker Kim1 in kidneys of control and Chmp1a+/− mice with or without cisplatin and liproxstatin injection. Sham-treated group: WT (n = 3), Chmp1a+/− (n = 3); cisplatin-treated group: WT (n = 5), Chmp1a+/− (n = 5); cisplatin and liproxstatin-treated group: WT (n = 4), Chmp1a+/− (n = 4). All data are represented as mean ± SEM. P value was calculated by two-way ANOVA with post hoc Tukey test for a, b, e, g, h, i. P value was calculated by two-tailed t-test for f. P < 0.05 is statistically significant. A Source Data file is available for this figure.

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