Fig. 4: Increased c-Fos activation in orexin neurons upon high-fat diet (HFD) feeding and improved glucose tolerance in Ox2R∆SERT mice fed a HFD. | Nature Communications

Fig. 4: Increased c-Fos activation in orexin neurons upon high-fat diet (HFD) feeding and improved glucose tolerance in Ox2R∆SERT mice fed a HFD.

From: Orexin receptors 1 and 2 in serotonergic neurons differentially regulate peripheral glucose metabolism in obesity

Fig. 4

a Representative images of RNAscope in situ hybridization in the lateral hypothalamus (LH) of a BL/6 mouse fed a control diet (CD) or HFD. n = 5 (CD) and 4 (HFD). b Percentages of c-Fos positive neurons in orexin neurons. n = 5 (CD) and 4 (HFD). p = 0.029. c, g Average body weight, glucose tolerance test under d, h 16-h and e, i 6-h fasting conditions, and f, j insulin tolerance test of control (Ctrl) and Ox1R∆SERT mice on normal chow diet (NCD) or HFD. NCD-Ctrl and NCD-Ox1R∆SERT, n = 9; HFD-Ctrl, n = 8 (g, h, j) or 11 (i); HFD-Ox1R∆SERT, n = 10 (g, h, j) or 12 (i). i p = 0.16 at 30 min. k, o Average body weight, glucose tolerance test under l, p 16-h and m, q 6-h fasting conditions, and n, r insulin tolerance test of Ctrl and Ox2R∆SERT mice on NCD or HFD. NCD-Ctrl, n = 11; NCD-Ox2R∆SERT, n = 8; HFD-Ctrl, n = 11 (o, p, r) or 13 (q); HFD-Ox2R∆SERT, n = 9. p p = 0.0002 (30 min) and 0.045 (60 min); q p = 0.076 (30 min). Magenta, orexin; yellow, c-Fos. Scale bar: 100 µm. Data are represented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; as determined by unpaired two-tailed Student’s t-test (b) or two-way ANOVA followed by Sidak’s post hoc test (i, p, q). Two-way ANOVA revealed a significant main effect of genotype in (i) (F (1, 105) = 7.86, p = 0.0060), p (F (1, 90) = 18.22, p < 0.0001) and q (F (1, 100) = 6.08, p = 0.015). Source data are provided as a Source Data file.

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