Fig. 5: Insulin sensitivity is impaired in Ox1R∆SERT mice but improved in Ox2R∆SERT mice fed a HFD in hyperinsulinemic-euglycemic clamp analysis. | Nature Communications

Fig. 5: Insulin sensitivity is impaired in Ox1R∆SERT mice but improved in Ox2R∆SERT mice fed a HFD in hyperinsulinemic-euglycemic clamp analysis.

From: Orexin receptors 1 and 2 in serotonergic neurons differentially regulate peripheral glucose metabolism in obesity

Fig. 5

a Clamp glucose infusion rates (GIR), b hepatic glucose production (HGP) in the basal state and during the steady state of clamp analysis, and c tissue-specific insulin-stimulated [1-14C]-Deoxy-D-glucose uptake in white adipose tissue (WAT), brown adipose tissue (BAT), and skeletal muscle (SM) under steady-state conditions of control (Ctrl) and Ox1R∆SERT mice. HFD-Ctrl, n = 8; HFD-Ox1R∆SERT, n = 14. a p = 0.0025 (110 min) and 0.0028 (120 min); c p = 0.011 (BAT) and 0.0070 (SM). d Clamp GIR, e HGP in the basal state and during the steady state of clamp analysis, and f tissue-specific insulin-stimulated [1-14C]-Deoxy-D-glucose uptake in WAT, BAT, and SM under steady-state conditions of control (Ctrl) and Ox2R∆SERT mice. HFD-Ctrl, n = 10; HFD-Ox2R∆SERT, n = 11. d p = 0.034 (90 min), 0.047 (100 min), 0.041 (110 min) and 0.042 (120 min); e p = 0.048. Data are represented as means ± SEM. *p < 0.05, **p < 0.01; as determined by two-way ANOVA followed by Sidak’s post hoc test (a, d) or unpaired two-tailed Student’s t-test (c, e). Two-way ANOVA revealed a significant main effect of genotype in (a) (F (1, 220) = 18.30, p < 0.0001) and (d) (F (1, 209) = 35.39, p < 0.0001). Source data are provided as a Source Data file.

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