Fig. 6: Insulin signaling in liver and BAT in obese Ox1R^ΔSERT and Ox2R^ΔSERT mice.

a, b Western blot images and quantification of p-Akt^Ser473, Akt, and G6Pase protein in the liver of control and Ox1R^∆SERT mice. HFD-Ctrl, n = 8; HFD-Ox1R^∆SERT, n = 14. c, d Western blot images and quantification of p-Akt^Ser473, Akt, and G6Pase protein in the liver of control and Ox2R^∆SERT mice. HFD-Ctrl, n = 10; HFD-Ox2R^∆SERT, n = 11. p = 0.013 (p-Akt^Ser473) and 0.0085 (G6Pase). Data are represented as means ± SEM. *p < 0.05, **p < 0.01; as determined by unpaired two-tailed Student’s t-test. e Volcano plot of differential expression analysis of RNA sequencing of BAT in Ox1R^∆SERT mice, compared to control mice. Some genes of interest are annotated. The differential gene expression test was done using negative binomial generalized linear models implemented in DESeq2 1.26.0. P-values are false discovery rates adjusted using the Benjamini-Hochberg procedure. f Top 15 differentially regulated gene ontology (GO) terms of class biological process in BAT of Ox1R^∆SERT mice, compared to control mice. Significance is mapped to color, the dot size represents the number of significant genes in the GO term and the x-axis maps the percentage of significant genes to the overall gene GO term size. Gene-ontology term analysis of the 265 differentially expressed genes was carried out using the clusterProfiler R package, which utilizes an over-representation analysis calculating p-values by hypergeomtric distributions. P-values are FDR-adjusted. n = 4. Source data are provided as a Source Data file.