Fig. 1: NISDA assay for rapid detection of SARS-CoV-2 RNA. | Nature Communications

Fig. 1: NISDA assay for rapid detection of SARS-CoV-2 RNA.

From: RETRACTED ARTICLE: A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Fig. 1

The reaction mixture contains three key components, including a DNA duplex (the Initiator), and two DNA molecular beacon structures (probe M1 and probe M2). In presence of viral RNA/DNA, toehold-mediated template displacement and cascade signal amplification occur sequentially, following by fluorescence detection after 30 min at 42 °C. INA is intercalating nucleic acid with enhanced binding affinity. F and Q denote for 6-Fam fluorophore and bhq-1 quencher, respectively. Letter labels denote for domains. The domains labeled with primes are complementary sequences.

Back to article page