Fig. 2: Structure analysis of the designed probes. | Nature Communications

Fig. 2: Structure analysis of the designed probes.

From: RETRACTED ARTICLE: A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Fig. 2

PAGE (12% TBM) analysis of a, amplification and b, displacement parts of the NISDA assay for N gene. a, b Investigating the role of Template length on the occurrence of the cascade reaction (amplification); decreasing the Template length from 30 to 22 nt resulted in disappearing of the unwanted bands and emergence of a new bands corresponding to the target M1:M2 duplex and recycling of the Template. c Typical gel image of the Initiator (T/INAt_15) before and after the PAGE purification together with the PAGE analysis on the displacement of Template by mimic SARS-CoV-2 (85 nt) strand. T denotes for Template. All the molecular weight markers are 100 bp. Each experiment was repeated at least two times independently.

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