Fig. 3: Assay performance on the Template. | Nature Communications

Fig. 3: Assay performance on the Template.

From: RETRACTED ARTICLE: A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Fig. 3

a Genome map of the SARS-CoV-2 isolate Wuhan-Hu-1, depicting two studied regions of RdRP and N genes and their corresponding nucleotide positions (red bars). Typical curves illustrating the kinetics of the fluorescence signal of the assay, designed for b, RdRP gene and c, N gene, over time for serial dilutions of the Template from 300 nM down to 300 aM in TES buffer (pH 7.8). The linear regression plots from 300 nM down to 3 fM are shown for each corresponding curves. Three independent experiments were run (n = 3). The assay comprising M1 and M2 probes was launched at the constant temperature of 42 °C while the fluorescence intensity of 6-FAM was recording each 3.5 min. RFU and Scr denote for relative fluorescence unit and scrambled DNA sequence, respectively. The Slope values (Ɵ) were calculated based on the value of saturated fluorescence intensity recorded for each graph (indicated as filled circles) dividing by the corresponding time intervals. Error bars represent mean ± SD. The solid boxes represent mean values for buffer ± SD. Source data for (b, c) are provided as a source data file.

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