Fig. 4: The role of Initiator in the Template displacement.

a Location of the toehold-binding sites for both RdRP and N genes. Typical curves illustrating the fluorescence signal kinetics of the NISDA assay over time, designed for b, RdRP gene and c, N gene using different Initiators of T/DNAt-15 (solid blue circle), T/INAt-8 (hollow red circle), and T/INAt-15 (hollow green square). Water (hollow black triangle) means no Initiator, only M1 and M2. Source data for (b, c) are provided as a source data file.