Fig. 4: Identification of distinct gene lengthening patterns and genes responsible for overall data trends across embryonic ages.
From: The landscape of alternative polyadenylation in single cells of the developing mouse embryo
a Heatmap of mean differences in 3′-UTR lengths for 5169 genes with significant differences in PAS usage across embryonic stages. Heatmap is column-centered and clustered by Pearson correlation as a distance metric. b Schematic of IIR plot visualization using PAS counts for each of five embryonic stages. Vertical red lines along the 3′-UTR indicate PASs that are significantly different between stages by the χ2 test (P < 0.05). c IIR plots for six genes among representative clusters shown in (a), and colored by embryonic stage. See also Supplementary Fig. 5 for clustering according to differences in entropy. See also Supplementary Data 3 for a table of read counts associated with each PAS for each gene and embryonic stage.
