Fig. 2: PTENα promotes tumor immune escape.

a–g Cancer vaccination model of Mock or PTENα transfected Pten^−/− B16 cells. a Schematic diagram of the cancer vaccine model. b Tumor volumes of C57BL/6 mice subjected to the cancer vaccination model were monitored overtime. The unimmunized group refers to mice without irradiated B16 cells immunization. The statistical significances between the immunized groups were indicated (PTENα-unimmunized, n = 4 mice; other groups, n = 5 mice, mean ± s.e.m., *P (d16) = 0.0254, *P (d20) = 0.0339, *P (d22) = 0.0487, *P (d24) = 0.0450, **P = 0.0077). c The tumor-infiltrating lymphocytes (TILs) were isolated when the tumor volumes of the Mock-immunized group reach 200 mm³, and the cells were subjected to flow cytometry analysis. The cell counts of CD45⁺ cells were used for statistical analysis (n = 4 mice, mean ± SD, **P = 0.0044). Gating strategies were shown in Supplementary Fig. 3b. d–g TILs were isolated when the tumor volumes of Mock-immunized group reach 200 mm³, and CD45⁺ cells were sorted, using for 10× scRNA-seq with two replicates. 25,098 cells were identified as 12 clusters utilizing graph-based clustering. d Cd8T cells were further analyzed and identified as 4 clusters utilizing graph-based clustering. e Violin plot of differentiated genes in the 4 clusters of Cd8T cells. The color of each violin is the average expression value of a given gene. f and g Umap and proportion of the 4 clusters of Cd8T cells were shown. h and i Adoptive T cell transfer model of Mock or PTENα expressing B16-Pten^−/− cells. h An illustration of mice treatment. i Tumor volumes was monitored over time (Mock, n = 6 mice; PTENα, n = 7 mice, mean ± s.e.m., *P (d18) = 0.0360, *P (d22) = 0.0481). Statistical significances between CD8⁺ T cells transferred groups were shown. Statistical significance was assessed by a two-tailed unpaired Student’s t test without multiple comparisons test (b, c, i). Data are representative of two (b and i) or three (c) independent experiments with similar results. Source data are provided as a Source Data file. See also Supplementary Figs. 2 and 3.