Fig. 3: Multigenerational cage trials demonstrating efficient population suppression.
From: Suppressing mosquito populations with precision guided sterile males
A To generate sufficient mosquito numbers, three lines were raised separately, including homozygous Cas9, double-homozygous gRNAβTub + myo-fem, and WT. To generate pgSIT progeny, virgin Cas9 ♀’s were genetically crossed to gRNAβTub + myo-fem ♂’s, and eggs were collected. B To perform multigenerational population cage trials of pgSIT, two strategies were employed: release of eggs (B, top panel); release of mature adults (B, bottom panel). For both strategies, multiple pgSIT:WT release ratios were tested, including: 1:1, 5:1, 10:1, 20:1, and 40:1. Each generation, total eggs were counted, and 100 eggs were selected randomly to seed the subsequent generation. The remaining eggs were hatched to measure hatching rates and score transgene markers. This procedure was repeated after each generation until each population was eliminated (Supplementary Table 16). C Multigenerational population cage data for each release threshold plotting the proportion of eggs hatched each generation. Source data are provided as a Source Data file.
