Fig. 7: ATP-fueled transient self-sorting DNTs by orthogonal direct tile activation (Strategy 2).
From: Autonomous DNA nanostructures instructed by hierarchically concatenated chemical reaction networks
a Schematic representation of ATP-fueled, transiently self-sorting DNT self-assemblies. b Time-dependent CLSM measurements of ATP-fueled transient self-sorting DNTs. Scale bar: 10 μm. c Time-dependent < L > s and yields of the both DNTs measured from CLSM images. Error bars correspond to the standard deviation of at least three random fields of view each with an area of 185.4 × 185.4 μm2 from duplicate experiments. Conditions: 25 °C, 1 μM Inactive tile 2 (with 1 μM Inhibitor 2), 1 μM Inactive tile 3 (with 1 μM Inhibitor 3), 10 μM Complex 1, 5 μM Input 5, 5 μM Input 6, 0.8 WU μL−1 T4 DNA ligase, 1.5 U μL−1 BsaI, and 16 μM ATP.
