Fig. 5: Kinetic HDX-MS data of WT CD47BRIL and mutants.
From: Structure of the human marker of self 5-transmembrane receptor CD47
a The HDX levels of WT CD47BRIL and mutants are color coded and mapped onto the crystal structure of full-length CD47BRIL-B6H12 complex (Fab B6H12 atoms omitted). Regions that were not identified in the HDX-MS experiments are colored gray. b Close view of the ECLR showing deuterium uptake at 60 min. c Deuterium uptake plots of peptides corresponding to the inter-domain 114RVSSWF119 linker region, ECL1 and helix III, ECL2 and the EC portion of helix V. d Deuterium uptake plots of WT CD47BRIL and mutants for peptides covering the 114RVSSWF119 linker region. Each data-point is presented as mean values across two replicates. Error bars represent mean values ± SD of duplicate measurements. At least two independent experiments were performed with a panel a receptor constructs. The kinetic differential HDX data are provided in Supplementary Data 5.
