Table 3 EM statistics.
Spike C5 (PDB ID 7OAN, EMD-12777) | |
|---|---|
Data collection and processing | |
Magnification | 81,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 50 |
Defocus range (μm) | 1.0–3.0 |
Pixel size (Å/pix) (super resolution) | 0.53 |
Symmetry imposed | C3 |
Initial particle images (no.) | 1,061,364 |
Final particle images (no.) | 227,898 |
Map resolution (Å) | 2.9 |
FSC threshold | 0.143 |
Map resolution range (Å) | 2.7–6.7 |
Refinement | |
Initial model used | 6VXX |
Model resolution (Å) | 3.0 |
FSC threshold | 0.143 |
Model resolution range (Å) | 198.2–3.0 |
Map sharpening B factor (Å2) | −118 |
Model composition | |
Non-hydrogen atoms | 28,218 |
Protein residues | 3510 |
B factors (Å2) | |
Protein | 121 |
R.m.s. deviations | |
Bond lengths (Å) | 0.011 |
Bond angles (°) | 1.241 |
Validation | |
MolProbity score | 1.84 |
Clashscore | 8.13 |
Poor rotamers (%) | 1.35 |
Ramachandran plot | |
Favoured (%) | 95.75 |
Allowed (%) | 4.08 |
Disallowed (%) | 0.17 |
