Fig. 4: Measurement of anti-viral IgG responses using a COVID-19 viral array.
From: New-onset IgG autoantibodies in hospitalized patients with COVID-19
a Heatmap depicting IgG antibodies using a 28-plex bead-based protein array. Viral protein antigens are grouped based on sixteen proteins from SARS-CoV-2 (left panel), other coronaviruses (middle panel), and other viruses (right panel), labeled on the x-axis. Most recombinant viral proteins were engineered to include a 6X-His-tag, which was used to validate conjugation to beads using an anti-epitope monoclonal antibody (bottom of the panel). The same COVID-19 patients from Fig. 3 (see Supplementary Figures 9 and 10) were analyzed (top panel, n = 94 longitudinal COVID-19 samples, including paired samples from 44 subjects and 2 subjects who had 3 available time points each, subjects UP70 and UP71). HC (n = 16, middle panel). Two patient sample pairs (UP63 and UMR20) were excluded from analysis due to technical failure on the viral array assay. Colors correspond to the MFI values shown at right. b Heatmap depicting statistically significant anti-viral IgG responses. Colors indicate IgG antibodies whose MFI measurements are >5 SD (red) or <5 SD (black) above the average MFI for HC samples collected prior to the COVID-19 pandemic. Source data are provided as a Source Data file.
